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MGST1 was up-regulated, but ALOX5 was down-regulated in DDP-resistant NSCLC samples and cells

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Microsomal glutathione transferase 1 confers cisplatin resistance of non-small cell lung cancer via interaction with arachidonate lipoxygenase 5 to repress ferroptosis

doi: 10.22038/ijbms.2024.79203.17160

Figure Lengend Snippet: MGST1 was up-regulated, but ALOX5 was down-regulated in DDP-resistant NSCLC samples and cells

Article Snippet: The membranes were blocked with 5% skim milk for one hour and then incubated with primary antibodies against MGST1 (PA5-79670, 1:1000, Thermo Fisher), ALOX5 (#3289, 1:1000, CST, USA), glutathione peroxidase4 ( GPX4, A21440, 1:500, ABclonal, Wuhan, China), solute carrier family 7 member 11 (SLC7A11, A2413, 1:500, ABclonal), Acyl-CoA synthetase (ACSL4, A20414, 1:1000, ABclonal), and reduced glyceraldehyde-phosphate dehydrogenase (GAPDH, A19056, 1:10000, ABclonal) overnight at 4 ° C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (AS014, 1:2000, ABclonal) for one hour.

Techniques:

MGST1 led to ferroptosis inhibition in NSCLC cells via direct interaction with ALOX5

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Microsomal glutathione transferase 1 confers cisplatin resistance of non-small cell lung cancer via interaction with arachidonate lipoxygenase 5 to repress ferroptosis

doi: 10.22038/ijbms.2024.79203.17160

Figure Lengend Snippet: MGST1 led to ferroptosis inhibition in NSCLC cells via direct interaction with ALOX5

Article Snippet: The membranes were blocked with 5% skim milk for one hour and then incubated with primary antibodies against MGST1 (PA5-79670, 1:1000, Thermo Fisher), ALOX5 (#3289, 1:1000, CST, USA), glutathione peroxidase4 ( GPX4, A21440, 1:500, ABclonal, Wuhan, China), solute carrier family 7 member 11 (SLC7A11, A2413, 1:500, ABclonal), Acyl-CoA synthetase (ACSL4, A20414, 1:1000, ABclonal), and reduced glyceraldehyde-phosphate dehydrogenase (GAPDH, A19056, 1:10000, ABclonal) overnight at 4 ° C, followed by incubation with horseradish peroxidase-conjugated secondary antibody (AS014, 1:2000, ABclonal) for one hour.

Techniques: Inhibition